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Thiobarbituric acid-reacting substances derived from autoxidizing linoleic and linolenic acids

Authors :
J.M.C. Gutteridge
J. Stocks
T.L. Dormandy
Source :
Analytica chimica acta. 70(1)
Publication Year :
1974

Abstract

Thin-layer chromotographic techniques are described for the preparation and separation of water-soluble compounds formed by the autoxidation of pure linoleic and linolenic acids. Location of 15 main fractions with Schiffs reagent on fibreglass sheets was possible. These could be eluted for biological testing and chromogenic behaviour with thiobarbituric acid reagent. At least 7 bands from autoxidized linoleic acid and 9 from autoxidized linolenic acid had peak absorbance maxima at 532 nm identical to that characteristically given by malonyldialdehyde (MDA). Possible reactions and interpretations for the MDA test are discussed.

Details

ISSN :
00032670
Volume :
70
Issue :
1
Database :
OpenAIRE
Journal :
Analytica chimica acta
Accession number :
edsair.doi.dedup.....3b0ea4834be5eec81cfe44ed43f26bb1