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Thiobarbituric acid-reacting substances derived from autoxidizing linoleic and linolenic acids
- Source :
- Analytica chimica acta. 70(1)
- Publication Year :
- 1974
-
Abstract
- Thin-layer chromotographic techniques are described for the preparation and separation of water-soluble compounds formed by the autoxidation of pure linoleic and linolenic acids. Location of 15 main fractions with Schiffs reagent on fibreglass sheets was possible. These could be eluted for biological testing and chromogenic behaviour with thiobarbituric acid reagent. At least 7 bands from autoxidized linoleic acid and 9 from autoxidized linolenic acid had peak absorbance maxima at 532 nm identical to that characteristically given by malonyldialdehyde (MDA). Possible reactions and interpretations for the MDA test are discussed.
- Subjects :
- Autoxidation
Chemical Phenomena
Linolenic Acids
Thiobarbituric acid
Elution
Chromogenic
Linolenic acid
Linoleic acid
Thiones
Biochemistry
Analytical Chemistry
Absorbance
chemistry.chemical_compound
Chemistry
chemistry
Linoleic Acids
Reagent
Barbiturates
Environmental Chemistry
Organic chemistry
Indicators and Reagents
Chromatography, Thin Layer
Oxidation-Reduction
Spectroscopy
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 70
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Analytica chimica acta
- Accession number :
- edsair.doi.dedup.....3b0ea4834be5eec81cfe44ed43f26bb1