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Molecular cloning of a house dust mite allergen with common antibody binding specificities with multiple components in mite extracts
- Source :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 23(11)
- Publication Year :
- 1993
-
Abstract
- Plaque radio-immuno assay has been used to isolate an IgE-binding clone from a lambda gt11 library of Dermatophagoides pteronyssinus cDNA. The clone HD6 contained DNA encoding a 215 residue protein which contained a predicted 17 amino acid residue leader sequence, no cysteines and a single N-glycosylation site. The 198 residue mature protein would have a predicted MW of 22,177 D. No homologues were found in searches of the data banks. Sera from 14/38 allergic children reacted strongly with the polypeptide produced by the clone (37%). Skin tests showed reactivity in 16/30 (53%) allergic patients and 0/10 of controls. Affinity purification of rabbit antibodies with the clone showed that antibodies to the polypeptide had specificities to multiple products in mite extracts corresponding to components of Mr 29, 27 and 24 K by Western blotting. Absorption studies of IgE in allergic serum indicated further entities at 13 and 11.5 kD. It is proposed to name this allergen Der p VII.
- Subjects :
- Hypersensitivity, Immediate
DNA, Complementary
Glycosylation
Immunology
Blotting, Western
Molecular Sequence Data
Clone (cell biology)
Molecular cloning
Protein Sorting Signals
medicine.disease_cause
Immunoglobulin E
Allergen
Affinity chromatography
Antibody Specificity
Complementary DNA
medicine
Immunology and Allergy
Animals
Humans
Amino Acid Sequence
Antigens, Dermatophagoides
Cloning, Molecular
Child
Peptide sequence
Glycoproteins
Skin Tests
Mites
biology
Base Sequence
Tissue Extracts
Allergens
Molecular biology
biology.protein
Rabbits
Antibody
Protein Processing, Post-Translational
Subjects
Details
- ISSN :
- 09547894
- Volume :
- 23
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
- Accession number :
- edsair.doi.dedup.....3ae723380a66b789da0311be1dcf7997