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Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle: distance of PCM during oocyte maturation
- Source :
- Journal of assisted reproduction and genetics. 28(2)
- Publication Year :
- 2010
-
Abstract
- To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes.FSH-induced oocytes matured in Waymouth's MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression.The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition.These results indicate that the 'maturation competence' of MII oocytes can be evaluated on the basis of the distance between PCMs.
- Subjects :
- Integrins
Gene Expression
Fertilization in Vitro
Spindle Apparatus
Biology
Oogenesis
Mice
Meiosis
Gamete Biology
Genetics
medicine
Animals
Blastocyst
Metaphase
Genetics (clinical)
Centrosome
Meiotic spindle organization
urogenital system
Obstetrics and Gynecology
General Medicine
Miosis
Oocyte
Cell biology
In vitro maturation
medicine.anatomical_structure
Reproductive Medicine
Oocytes
Female
Follicle Stimulating Hormone
Developmental Biology
Subjects
Details
- ISSN :
- 15737330
- Volume :
- 28
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of assisted reproduction and genetics
- Accession number :
- edsair.doi.dedup.....3ac416eb68a344b149f0dee4271c9e26