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Cell-cycle regulation of human B-myb transcription
- Source :
- Gene. 160(2)
- Publication Year :
- 1995
-
Abstract
- We demonstrate here that activity of the human B-myb promoter is regulated during the cell cycle by the E2 transcription factor (E2F). Comparison of the human B-myb promoter sequence with that of its murine counterpart revealed an evolutionally conserved sequence that contains an E2F-binding site. In transiently transfected murine NIH3T3 and human HaCaT cells, luciferase (Luc) reporter activity directed by the human B-myb promoter was found to increase significantly in late G1/S phase of the cell cycle. Mutation of the promoter E2F site resulted in significantly greater Luc activity in NIH3T3 and HaCaT cells made quiescent by serum deprivation, indicating that E2F repressed transcription of this gene during G0. Analysis of E2F DNA-binding activity in G0 HaCaT cells revealed a distinct complex that apparently contained neither the retinoblastoma gene protein, pRb, nor the related p107 protein. De-repression of transcription in S phase was accompanied by the disappearance of this G0 E2F complex and the appearance of a distinct complex containing p107. In addition, complexes containing pRb were detected at both stages of the cell cycle.
- Subjects :
- Keratinocytes
Transcription, Genetic
Recombinant Fusion Proteins
Response element
Molecular Sequence Data
Cell Cycle Proteins
Resting Phase, Cell Cycle
S Phase
Mice
Sp3 transcription factor
Sequence Homology, Nucleic Acid
Genetics
E2F1
Animals
Humans
E2F
Promoter Regions, Genetic
Transcription factor
Phylogeny
Cell Line, Transformed
General transcription factor
biology
Base Sequence
Cell Cycle
Retinoblastoma protein
Promoter
General Medicine
3T3 Cells
Molecular biology
E2F Transcription Factors
DNA-Binding Proteins
Gene Expression Regulation
biology.protein
Trans-Activators
biological phenomena, cell phenomena, and immunity
Carrier Proteins
Sequence Alignment
Transcription Factor DP1
Retinoblastoma-Binding Protein 1
Transcription Factors
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 160
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....3abadfac51caf357d5a58e344f5f7c94