Back to Search
Start Over
Thiazide-sensitive NaCl absorption in rat cortical collecting duct
- Source :
- American Journal of Physiology-Renal Physiology. 259:F519-F528
- Publication Year :
- 1990
- Publisher :
- American Physiological Society, 1990.
-
Abstract
- The mechanism of transepithelial NaCl transport was investigated in isolated perfused cortical collecting ducts from the kidneys of deoxycorticosterone-treated rats. In the presence of vasopressin, hydrochlorothiazide (0.1 mM) markedly reduced the net rate of Na absorption, Cl absorption, and active fluid absorption but did not significantly change the transepithelial voltage. Similarly, in the absence of vasopressin, hydrochlorothiazide decreased the rate of sodium absorption by 50% without affecting transepithelial voltage. Amiloride (30 microM) completely eliminated the lumen-negative voltage but decreased net sodium absorption only by approximately 50%. In the presence of amiloride, chloride absorption occurred against an electrochemical gradient for chloride, indicating that there was active chloride absorption. Bumetanide (0.1 mM) did not affect chloride absorption or spontaneous fluid absorption in the presence of vasopressin. The combination of amiloride and hydrochlorothiazide inhibited net sodium absorption by a greater extent than did either agent alone. These results demonstrate the presence of the following two parallel sodium transport pathways in cortical collecting ducts from mineralocorticoid-replete rats: 1) an electrogenic pathway blocked by amiloride, which presumably involves an apical sodium channel, and 2) a thiazide-inhibitable electroneutral pathway, which presumably utilizes apical Na-Cl cotransport and mediates secondary active transport of chloride.
- Subjects :
- Male
Vasopressin
medicine.medical_specialty
Kidney cortex
Kidney Cortex
Physiology
Sodium Chloride
Absorption
Amiloride
Chlorides
Internal medicine
medicine
Animals
Nacl absorption
Kidney Tubules, Collecting
Ouabain
Bumetanide
Thiazide
Chemistry
Sodium
Biological Transport
Rats, Inbred Strains
Rats
Hydrochlorothiazide
Kidney Tubules
Endocrinology
medicine.drug
Kidney tubules
Subjects
Details
- ISSN :
- 15221466 and 1931857X
- Volume :
- 259
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Renal Physiology
- Accession number :
- edsair.doi.dedup.....3aa078faf52431c90fc6c83d53918b4b