Translation Analysis at the Genome Scale by Ribosome Profiling

Authors :: Olivier Namy
Rachel Legendre
Agnès Baudin-Baillieu
Isabelle Hatin
Institut de Biologie Intégrative de la Cellule (I2BC)
Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Institut de Biologie Intégrative de la Cellule ( I2BC )
Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 )
Source :: Yeast Functional Genomics, Yeast Functional Genomics, pp.105-124, 2016, 9781493930784. ⟨10.1007/978-1-4939-3079-1_7⟩, Methods in Molecular Biology ISBN: 9781493930784, Yeast Functional Genomics, pp.105-124, 2016, 9781493930784. 〈10.1007/978-1-4939-3079-1_7〉
Publication Year :: 2016
Publisher :: HAL CCSD, 2016.
Abstract: International audience; Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.

Language :: English
ISBN :: 978-1-4939-3078-4
ISBNs :: 9781493930784
Database :: OpenAIRE
Journal :: Yeast Functional Genomics, Yeast Functional Genomics, pp.105-124, 2016, 9781493930784. ⟨10.1007/978-1-4939-3079-1_7⟩, Methods in Molecular Biology ISBN: 9781493930784, Yeast Functional Genomics, pp.105-124, 2016, 9781493930784. 〈10.1007/978-1-4939-3079-1_7〉
Accession number :: edsair.doi.dedup.....3a9bf2c257be70dd006ab9c6da3b4925
Full Text :: https://doi.org/10.1007/978-1-4939-3079-1_7⟩