Back to Search
Start Over
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013
- Source :
- Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-16 (2020), Stem Cell Research & Therapy
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Background Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5 in chondrogenic differentiation function in MSCs. Methods Stem cells from apical papilla (SCAPs) were used. The Alcian Blue staining, pellet culture system, and cell transplantation in rabbit knee cartilage defect were used to evaluate the chondrogenic differentiation function of MSCs. Western blot, real-time RT-PCR, and ChIP assays were used to evaluate the molecular mechanisms. Results DLX5 and HOXC8 expressions were upregulated during chondrogenic differentiation. In vitro results showed that DLX5 and HOXC8 enhanced the expression of chondrogenic markers including collagen II (COL2), collagen V (COL5), and sex-determining region Y box protein 9 (SOX9) and promoted the chondrogenic differentiation and the formation of cartilage clumps in the pellet culture system. Mechanically, DLX5 and HOXC8 formed protein complexes and negatively regulated the LncRNA, LINC01013, via directly binding its promoter. In vivo transplantation experiment showed that DLX5 and HOXC8 could restore the cartilage defect in the rabbit knee model. In addition, knock-down of LINC01013 enhanced the chondrogenic differentiation of SCAPs. Conclusions In conclusion, DLX5 and HOXC8 enhance the chondrogenic differentiation abilities of SCAPs by negatively regulating LINC01013 in SCAPs, and provided the potential target for promoting cartilage tissue regeneration.
- Subjects :
- Medicine (miscellaneous)
SOX9
Biochemistry, Genetics and Molecular Biology (miscellaneous)
lcsh:Biochemistry
Western blot
HOXC8
medicine
Animals
lcsh:QD415-436
DLX5
Cells, Cultured
lcsh:R5-920
medicine.diagnostic_test
Chondrogenic differentiation
Chemistry
Stem Cells
Research
Cartilage
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Chondrogenesis
LncRNA
Cell biology
Transplantation
medicine.anatomical_structure
embryonic structures
Molecular Medicine
Rabbits
Stem cell
lcsh:Medicine (General)
Stem cells from apical papilla (SCAPs)
Subjects
Details
- ISSN :
- 17576512
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....3a89b2a3c58c4e3ac85453f8a79c2377