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Influence of metal ions on human lymphocytes and the generation of titanium-specific T-lymphocytes
- Publication Year :
- 2011
-
Abstract
- Background: There is mounting evidence to suggest the involvement of the immune system by means of activation by metal ions released via biocorrosion, in the pathophysiologic mechanisms of aseptic loosening of orthopedic implants. However, the detailed mechanisms of how metal ions become antigenic and are presented to T-lymphocytes, in addition to how the local inflammatory response is driven, remain to be investigated. Methods: Human T-lymphocytes were cultured in the presence of a variety of metal ions before investigating functional and phenotypic changes using flow cytometric analysis. Additionally, human monocyte-derived dendritic cells (mDC) loaded with metal ions were used as antigen-presenting cells and incubated with naive T-lymphocytes with the aim of generating titanium-specific T-lymphocytes. Results: Using an autologous in vitro model, with mDC treated with Titanium (IV), we were able to induce Titanium (IV)-specific T-lymphocytes. These T-lymphocytes responded in a dose-related manner to Titanium (IV), while they did not cross-react with Titanium (III) or other metal ions, indicating that the new antigenic peptide complexes formed by Titanium (IV) are highly specific. Conclusion: This study showed that mDC exposed to Titanium (IV) are able to induce the generation of Titanium (IV)-specific T-lymphocytes, demonstrating the strong and specific antigenicity of Titanium (IV) ions released by biocorrosion.
- Subjects :
- Male
Antigenicity
Metal ions in aqueous solution
T-Lymphocytes
Inorganic chemistry
Biomedical Engineering
Biophysics
Aseptic loosening
Dose-Response Relationship, Immunologic
chemistry.chemical_element
2204 Biomedical Engineering
Bioengineering
610 Medicine & health
Monocytes
In vitro model
Biomaterials
Immune system
Antigen
Medicine
Humans
Antigens
Antigenic peptide
Cells, Cultured
Ions
Titanium
Antigen Presentation
1502 Bioengineering
business.industry
2502 Biomaterials
Dendritic Cells
Prostheses and Implants
equipment and supplies
10021 Department of Trauma Surgery
chemistry
Surgery
Female
business
Peptides
1304 Biophysics
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....3a8927a40f21b3ab1216f44a2d99596a