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Covalent cell surface functionalization of human fetal osteoblasts for tissue engineering
- Source :
- Bioconjugate Chemistry
-
Abstract
- The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne derivatives with cellular azido-expressing proteins. The incorporation via a tetraethylene glycol linker of a dipeptide and a reporter biotin allowed the proof of concept for the introduction of cell-specific peptide ligands and allowed us to follow the reaction in living cells. Tuning the conditions of the click reaction resulted in chemical functionalization of living human fetal osteoblasts with excellent cell survival.
- Subjects :
- multivalent linker
Stereochemistry
Biomedical Engineering
Pharmaceutical Science
Bioengineering
fetal cells
010402 general chemistry
01 natural sciences
cell survival
Cell membrane
chemistry.chemical_compound
Fetus
Biotin
Bone cell
medicine
Humans
human osteoblasts
Cells, Cultured
Pharmacology
Osteoblasts
Dipeptide
Tissue Engineering
010405 organic chemistry
Cell Membrane
Organic Chemistry
Membrane Proteins
0104 chemical sciences
medicine.anatomical_structure
chemistry
Alkynes
cell functionalization
Click chemistry
Biophysics
Surface modification
Click Chemistry
Bioorthogonal chemistry
click reaction
Hydrophobic and Hydrophilic Interactions
Linker
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Bioconjugate Chemistry
- Accession number :
- edsair.doi.dedup.....3a813c8b0ec17f56c61fd30bc02d691e