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The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells
- Source :
- BMC Neuroscience, BMC Neuroscience, Vol 10, Iss 1, p 13 (2009)
- Publisher :
- Springer Nature
-
Abstract
- Background Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem and progenitor cells. It is not known how these two signalling systems interact. Here, we examined the role of ECM components in regulating connexin expression and function in postnatal hippocampal progenitor cells. Results We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, and Cx47 mRNA and protein but only Cx32 and Cx36 mRNA are detected in distinct neural progenitor cell populations cultured in the absence of exogenous ECM. Multipotential Type 1 cells express Cx26, Cx30, and Cx43 protein. Their Type 2a progeny but not Type 2b and 3 neuronally committed progenitor cells additionally express Cx37, Cx40, and Cx45. Cx29 and Cx47 protein is detected in early oligodendrocyte progenitors and mature oligodendrocytes respectively. Engagement with a laminin substrate markedly increases Cx26 protein expression, decreases Cx40, Cx43, Cx45, and Cx47 protein expression, and alters subcellular localization of Cx30. These changes are associated with decreased neurogenesis. Further, laminin elicits the appearance of Cx32 protein in early oligodendrocyte progenitors and Cx36 protein in immature neurons. These changes impact upon functional connexin-mediated hemichannel activity but not gap junctional intercellular communication. Conclusion Together, these findings demonstrate a new role for extracellular matrix-cell interaction, specifically laminin, in the regulation of intrinsic connexin expression and function in postnatal neural progenitor cells.
- Subjects :
- Neurogenesis
Blotting, Western
Connexin
Cell Communication
Biology
Hippocampus
Connexins
lcsh:RC321-571
Extracellular matrix
03 medical and health sciences
Mice
Cellular and Molecular Neuroscience
0302 clinical medicine
Laminin
Extracellular
medicine
Animals
RNA, Messenger
Progenitor cell
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Cells, Cultured
030304 developmental biology
Mice, Knockout
Neurons
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Stem Cells
lcsh:QP351-495
Flow Cytometry
Molecular biology
Immunohistochemistry
Neural stem cell
Oligodendrocyte
Cell biology
Extracellular Matrix
Oligodendroglia
medicine.anatomical_structure
lcsh:Neurophysiology and neuropsychology
biology.protein
sense organs
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712202
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Neuroscience
- Accession number :
- edsair.doi.dedup.....3a7ab75f0b74fe760880a6ae85c35f70
- Full Text :
- https://doi.org/10.1186/1471-2202-10-13