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Antimicrobial resistance and genetic characterization of Shigella spp. in Shanxi Province, China, during 2006–2016
- Source :
- BMC Microbiology, Vol 19, Iss 1, Pp 1-11 (2019), BMC Microbiology
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Background Shigella spp., facultative anaerobic bacilli of the family Enterobacteriaceae, are one of the most common causes of diarrheal diseases in human worldwide which have become a significant public health burden. So, we aimed to analyze the antimicrobial phenotypes and to elucidate the molecular mechanisms underlying resistance to cephalosporins and fluoroquinolones in Shigella isolates from patients with diarrhea in Shanxi Province. Results During 2006–2016, we isolated a total of 474 Shigella strains (including 337 S. flexneri and 137 S. sonnei). The isolates showed high rates of resistance to traditional antimicrobials, and 26, 18.1 and 3.0% of them exhibited resistance to cephalosporins, fluoroquinolones and co-resistance to cephalosporins and fluoroquinolones, respectively. Notably, 91.1% of these isolates, including 22 isolates that showed an ACTSuT profile, exhibited multidrug resistance (MDR). The resistance rates to cephalosporins in S. sonnei isolates were higher than those in S. flexneri. Conversely, the resistance rates to fluoroquinolones were considerably higher in S. flexneri isolates. Among the 123 cephalosporins-resistant isolates, the most common extended-spectrum beta-lactamase gene was blaTEM-1, followed by blaCTX-M, blaOXA-1, and blaSHV-12. Six subtypes of blaCTX-M were identified, blaCTX-M-14 (n = 36) and blaCTX-M-55 (n = 26) were found to be dominant. Of all the 86 isolates with resistance to fluoroquinolones and having at least one mutation (Ser83Leu, His211Tyr, or Asp87Gly) in the the quinolone resistance-determining regions of gyrA, 79 also had mutation of parC (Ser80Ile), whereas 7 contained plasmid-mediated quinolone resistance genes including qnrA, qnrB, qnrS, and aac(60)-Ib-cr. Furthermore, pulsed-field gel electrophoresis analysis (PFGE) showed a considerable genetic diversity in S. flexneri isolates. However, the S. sonnei isolates had a high genetic similarity. Conclusions Coexistence of diverse resistance genes causing the emergence and transmission of MDR might render the treatment of shigellosis difficult. Therefore, continuous surveillance might be needed to understand the actual disease burden and provide guidance for shigellosis. Electronic supplementary material The online version of this article (10.1186/s12866-019-1495-6) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
medicine.medical_treatment
Resistance
lcsh:QR1-502
Drug resistance
medicine.disease_cause
lcsh:Microbiology
Drug Resistance, Multiple, Bacterial
MDR
Shigella
Child
Aged, 80 and over
0303 health sciences
Antiinfective agent
Middle Aged
Anti-Bacterial Agents
Child, Preschool
Female
Research Article
Fluoroquinolones
Plasmids
Adult
Microbiology (medical)
Shigellosis
China
Adolescent
Microbial Sensitivity Tests
Biology
Microbiology
Young Adult
03 medical and health sciences
Serotype
Antibiotic resistance
Bacterial Proteins
Drug Resistance, Bacterial
medicine
Pulsed-field gel electrophoresis
Humans
Aged
Dysentery, Bacillary
030306 microbiology
Infant
biochemical phenomena, metabolism, and nutrition
medicine.disease
bacterial infections and mycoses
Cephalosporins
Multiple drug resistance
Mutation
Beta-lactamase
Antimicrobial
Subjects
Details
- Language :
- English
- ISSN :
- 14712180
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology
- Accession number :
- edsair.doi.dedup.....3a70ce4d9cf0b69f1ea7c5a02a3a7de9