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Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice
- Source :
- The Journal of Nutritional Biochemistry. 41:25-33
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Mammalian siderophores are believed to play a critical role in maintaining iron homeostasis. However, the properties and functions of mammalian siderophores have not been fully clarified. In this study, we have employed Chrome Azurol S (CAS) assay which is a well-established method for bacterial siderophores study, to detect and quantify mammalian siderophores in urine samples. Our study demonstrates that siderophores in urine can be altered by diet, gut microbiota and inflammation. C57BL/6 mice, fed on plant-based chow diets which contain numerous phytochemicals, have more siderophores in the urine compared to those fed on purified diets. Urinary siderophores were up-regulated in iron overload conditions, but not altered by other tested nutrients status. Further, germ-free mice displayed 50% reduced urinary siderophores, in comparison to conventional mice, indicating microbiota biotransformation is critical in generating or stimulating host metabolism to create more siderophores. Altered urinary siderophores levels during inflammation suggest that host health conditions influence systemic siderophores level. This is the first report to measure urinary siderophores as a whole, describing how siderophores levels are modulated under different physiological conditions. We believe that our study opens up a new field in mammalian siderophores research and the technique we used in a novel manner has the potential to be applied to clinical purpose.
- Subjects :
- Male
0301 basic medicine
Siderophore
Iron Overload
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Urinary system
Clinical Biochemistry
Antibiotics
Siderophores
Inflammation
Urine
Gut flora
Diet, High-Fat
Biochemistry
Article
Selenium
03 medical and health sciences
0302 clinical medicine
Lipocalin-2
Biotransformation
medicine
Animals
Germ-Free Life
Hemochromatosis Protein
Molecular Biology
Crosses, Genetic
Mice, Knockout
Salmonella Infections, Animal
Nutrition and Dietetics
Anemia, Iron-Deficiency
biology
Vitamin A Deficiency
Metabolism
Colitis
biology.organism_classification
Diet
Gastrointestinal Microbiome
Mice, Inbred C57BL
030104 developmental biology
030220 oncology & carcinogenesis
Female
medicine.symptom
Biomarkers
Subjects
Details
- ISSN :
- 09552863
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- The Journal of Nutritional Biochemistry
- Accession number :
- edsair.doi.dedup.....3a684c9786a9026f5b545513000dcc14