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Transduction of recombinant human erythropoietin receptor cDNA into daughter progenitors derived from single CD343+ cord blood cells changes the differentiation profile of daughter progenitors
- Source :
- Journal of Leukocyte Biology. 63:389-394
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- In this study, we tested the capacity to change the differentiation profile of progenitor cells by retroviral-mediated transduction of EpoR cDNA into one of the paired daughter cells derived from single CD343+ CB cells. Our results show that for the non-viral-treated daughter cells, the majority (99.6%) formed the same colony type. However, with cells transduced with viral vectors, 7.1% of the daughter cells transduced with the EpoR cDNA formed either a burst forming unit-erythroid (BFU-E) or a colony-forming unit-granulocyte, macrophage, erythroid, megakaryocyte (CFU-GEMM) colony compared to the other daughter cell transduced with viral supernatant lacking EpoR cDNA, which formed either a colony-forming unit granulocyte-macrophage (CFU-GM) or a high proliferative potential-colony forming cell (HPP-CFC) colony. Expression of the transduced EpoR cDNA was confirmed in individual colonies by RT-PCR analysis. These results substantiate in a more rigorous fashion our previous results that it is possible to change the Epo-responsive differentiation profile of progenitor cells by transduction into these cells of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD343+ kit+ cells transduced with EpoR cDNA.
- Subjects :
- DNA, Complementary
Cell division
Genetic Vectors
Immunology
CD34
Antigens, CD34
Biology
Transfection
Polymerase Chain Reaction
Viral vector
Colony-Forming Units Assay
Transduction (genetics)
Megakaryocyte
Antigens, CD
Complementary DNA
Receptors, Erythropoietin
medicine
Humans
Immunology and Allergy
Progenitor cell
Cells, Cultured
Infant, Newborn
Cell Differentiation
Cell Biology
Fetal Blood
Hematopoietic Stem Cells
Molecular biology
Recombinant Proteins
Erythropoietin receptor
Retroviridae
medicine.anatomical_structure
embryonic structures
Cell Division
Subjects
Details
- ISSN :
- 19383673 and 07415400
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Leukocyte Biology
- Accession number :
- edsair.doi.dedup.....3a64394b7992bb61f542b48f8165239d