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Influence of dehydration on glycerophosphorylcholine and choline distribution along the rat nephron
- Source :
- Pflugers Archiv : European journal of physiology. 442(2)
- Publication Year :
- 2001
-
Abstract
- Glycerophosphorylcholine is one of the four major organic osmolytes in renal medullary cells, changing their intracellular osmolyte concentration in parallel with extracellular tonicity during cellular osmoadaptation. In this study, the tubular content of glycerophosphorylcholine was quantified in untreated and 48-h-dehydrated male rats. A chemiluminescence ultra-micromethod was developed to measure choline at the picomolar level in single tubules microdissected from collagenase-treated kidneys. The glycerophosphorylcholine level was calculated as the difference between total choline after acid hydrolysis and the free tubular choline content. In accordance with the glycerophosphorylcholine distribution pattern in different renal zones of untreated rats, low amounts of glycerophosphorylcholine were found in all cortical and outer medullary structures (
- Subjects :
- Male
medicine.medical_specialty
Physiology
Clinical Biochemistry
Nephron
Choline
Rats, Sprague-Dawley
chemistry.chemical_compound
Physiology (medical)
Internal medicine
medicine
Extracellular
Animals
Tissue Distribution
Kidney Tubules, Collecting
Receptor
Kidney Medulla
Dehydration
Chemistry
Nephrons
Glycerylphosphorylcholine
Rats
medicine.anatomical_structure
Endocrinology
Osmolyte
Tonicity
Sorbitol
Intracellular
Subjects
Details
- ISSN :
- 00316768
- Volume :
- 442
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Pflugers Archiv : European journal of physiology
- Accession number :
- edsair.doi.dedup.....3a5bd73bd4ed801dba22b52c3822bde4