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Post-conditioning preserves glycolytic ATP during early reperfusion: a survival mechanism for the reperfused heart
- Source :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 22(5-6)
- Publication Year :
- 2008
-
Abstract
- Glycolytic activity during the transition period from anaerobic to aerobic metabolism has been demonstrated to be critical for heart recovery in isolated reperfused hearts. The purpose of this work was to investigate the relevance of the glycolytic pathway in preserving the cardiac function of post-conditioned hearts.The activation of the glycolytic pathway in post-conditioned hearts was evaluated by measuring GLUT-4 insertion, glucose consumption and lactate production. Iodoacetic acid and 2-deoxy-D-glucose were administrated to the working hearts to evaluate the effect of glycolytic inhibition in the post-conditioning protective effect.Post-conditioning maneuvers applied to isolated rat hearts, after prolonged ischemia and before reperfusion, promoted recovery of cardiac mechanical function with sustained increase of GLUT-4 translocation and activation of the glycolytic pathway during ischemia and early reperfusion. Iodoacetate inhibited the protective effect of post-conditioning, without affecting the mitochondrial oxidative capacity. Glycolysis contribution to maintain mechanical function at early reperfusion was observed in post-conditioned hearts perfused with 2-deoxy-D-glucose and in hearts in which iodoacetate was administered only during reperfusion.It is concluded that in the post-conditioned heart, a functional compartmentation of anaerobic energy metabolism, at early reperfusion, plays a significant role in cardiac protection against reperfusion damage.
- Subjects :
- Male
medicine.medical_specialty
Phosphocreatine
Physiology
Cellular respiration
Post conditioning
Myocardial Reperfusion
Myocardial Reperfusion Injury
In Vitro Techniques
Adenosine Triphosphate
Cytosol
Sarcolemma
Internal medicine
Hexokinase
medicine
Animals
Glycolysis
Lactic Acid
Rats, Wistar
Glucose Transporter Type 4
Chemistry
Myocardium
Heart
Rats
Protein Transport
Endocrinology
Glucose
Anaerobic exercise
Subjects
Details
- ISSN :
- 14219778
- Volume :
- 22
- Issue :
- 5-6
- Database :
- OpenAIRE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Accession number :
- edsair.doi.dedup.....3a556acbaf059e5a1c52fed63c2089fc