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The effects of histamine and interleukin-6 on NGF release from cortical astrocytes in primary culture
- Source :
- Pflugers Archiv : European journal of physiology. 440(Suppl 1)
- Publication Year :
- 2016
-
Abstract
- The influence of neurotransmitter histamine and cytokine interleukin-6 (IL-6) on nerve growth factor (NGF) release was studied in rat neonatal cortical astrocytes in primary culture. Exposure of astrocytes to either histamine or IL-6 resulted in the stimulation of NGF release. Maximal stimulation of NGF release was obtained using histamine at concentration 100 nM after 24 h of treatment (2.3 fold increase over the basal secretion from the control cells). IL-6 (30 ng/ml) induced NGF secretion was 1.66 fold over the basal level. Time course of NGF release, after histamine or IL-6 treatment, showed elevation of NGF level in the culture medium after 8 h or 24 h, respectively. IL-6 antibody effectively blocked the IL-6 stimulatory effect on NGF release, but did not influence NGF release, evoked by histamine. IL-6 antibody alone did not show any influence on NGF release. Our results suggest that IL-6 and histamine stimulate release of NGF by two different and independent molecular pathways.
- Subjects :
- medicine.medical_specialty
Time Factors
Physiology
medicine.medical_treatment
Clinical Biochemistry
Stimulation
chemistry.chemical_compound
Physiology (medical)
Internal medicine
Nerve Growth Factor
medicine
Animals
Secretion
Rats, Wistar
Interleukin 6
Neurotransmitter
Receptor
Cells, Cultured
Cerebral Cortex
biology
Chemistry
Interleukin-6
Osmolar Concentration
Rats
Nerve growth factor
Cytokine
Endocrinology
nervous system
Astrocytes
biology.protein
Histamine
Subjects
Details
- ISSN :
- 14322013
- Volume :
- 440
- Issue :
- Suppl 1
- Database :
- OpenAIRE
- Journal :
- Pflugers Archiv : European journal of physiology
- Accession number :
- edsair.doi.dedup.....3a13255a2b2ae7d8c9defd6a2369813c