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Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests
- Source :
- Diagnostics, Vol 10, Iss 615, p 615 (2020), Diagnostics, Volume 10, Issue 9
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Human strongyloidiasis is an important soil-transmitted helminthiasis that affects millions worldwide and can develop into fatal systemic strongyloidiasis in immunosuppressed patients. We have developed two new rapid and simple-to-use immunochromatographic test (ICT) kits for rapid serodiagnosis that support stool examination for clinical diagnosis. Strongyloides stercoralis recombinant IgG immunoreactive antigen (GenBank: AAB97359.1<br />rSsIR-based ICT kit) was used for detection of IgG and IgG4 antibodies. The diagnostic efficacy of both kits was evaluated using human serum samples from strongyloidiasis patients, healthy individuals, and those with other parasitosis. At a prevalence of infection of 36.4%, the diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rSsIR-based IgG ICT kit were 91.7%, 83.8%, 76.4%, 94.6%, and 86.7%, respectively, and those of the rSsIR-based IgG4 ICT kit were 78.3%, 84.8%, 74.6%, 87.3%, and 82.4% respectively. The concordance between the two kits was 89.7%. The recombinant antigen can be produced to an unlimited extent and the kits can be used as point-of-care diagnostic tools and in large-scale surveys in endemic areas throughout tropical regions without necessitating additional facilities or ancillary supplies.
- Subjects :
- 0301 basic medicine
diagnosis
IgG
030231 tropical medicine
Clinical Biochemistry
Helminthiasis
Subclass
Strongyloides stercoralis
law.invention
03 medical and health sciences
0302 clinical medicine
rapid test
Antigen
law
recombinant antigen
parasitic diseases
medicine
strongyloidiasis
screening test
IgG4
lcsh:R5-920
biology
business.industry
immunochromatographic test
030108 mycology & parasitology
medicine.disease
biology.organism_classification
Strongyloidiasis
Strongyloides
Immunology
biology.protein
Recombinant DNA
Antibody
business
lcsh:Medicine (General)
Subjects
Details
- Language :
- English
- ISSN :
- 20754418
- Volume :
- 10
- Issue :
- 615
- Database :
- OpenAIRE
- Journal :
- Diagnostics
- Accession number :
- edsair.doi.dedup.....39f8b7610c3a895706441802b3d4098c