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RrS1-like Sequences of Water Frogs from Central Europe and Around the Aegean Sea: Chromosomal Organization, Evolution, Possible Function
- Source :
- Journal of Molecular Evolution. 72:368-382
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- RrS1-like sequences of water frogs (genus Pelophylax) display varied genomic organization, whereas the centromeric hybridization pattern reveals species-specific differences. Using fluorescent in situ hybridization, Pelophylax cf. bedriagae, Pelophylax kurtmuelleri, and Pelophylax ridibundus showed a hybridization signal at centromeres of chromosomes 1-5, but in P. kurtmuelleri the medium-small chromosome labeled was 10 rather than 8. Pelophylax cretensis had almost 16 of 26 centromeres labeled, as did Pelophylax lessonae from Poland when its chromosomes are hybridized with a homologous probe. When StuI-digested genomic DNA was hybridized with RrS1 probe, hybridization ladders for P. ridibundus from Poland have evenly spaced steps (about 100 bp) of uniform intensity from about 200 bp upward. Steps in hybridization ladders from circum-Aegean taxa vary in intensity: larger, odd-numbered steps are often fainter. A strong double band (800/900 bp) in Anatolian P. cf. bedriagae, emphasized by a weak 700 bp band, distinguishes them from P. kurtmuelleri from the Peloponnisos, in which the 900 bp band is almost absent. The ladder in P. cretensis lacks odd-numbered steps. A and B repeats, observed originally within the RrS1 satellite of P. ridibundus, occur also in the circum-Aegean frogs and in P. lessonae, Pelophylax epeiroticus, Pelophylax saharicus, and Pelophylax shqipericus. It is plausible that AB dimers or ABB trimers rather than A or B monomers correspond to functional/evolutionary units. The presence of regions similar to yeast CDEs and mammalian CENP-B boxes suggests a role for RrS1 sequences in centromere organization.
- Subjects :
- Male
Ranidae
Pelophylax
Centromere
Molecular Sequence Data
Sequence alignment
In situ hybridization
Chromosomes
Evolution, Molecular
Phylogenetics
Genetics
Animals
Molecular Biology
In Situ Hybridization, Fluorescence
Phylogeny
Ecology, Evolution, Behavior and Systematics
Genomic organization
Base Sequence
Geography
biology
Nuclear Proteins
Chromosome
biology.organism_classification
Europe
genomic DNA
Female
Centromere Protein B
Sequence Alignment
Subjects
Details
- ISSN :
- 14321432 and 00222844
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Evolution
- Accession number :
- edsair.doi.dedup.....39dfc281c0f81de9edcc79dcc409ea0e