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AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 109(38)
- Publication Year :
- 2012
-
Abstract
- The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein ( ApC/EBP ). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor synapses. However, the molecular mechanism underlying the rapid degradation of ApC/EBP transcript is not known. Here, we cloned an AU-rich element (ARE)-binding protein, ApAUF1, which functions as a destabilizing factor for ApC/EBP mRNA. ApAUF1 was found to bind to the 3′ UTR of ApC/EBP mRNA that contains AREs and subsequently reduces the expression of ApC/EBP 3′ UTR-containing reporter genes. Moreover, overexpression of ApAUF1 inhibited the induction of ApC/EBP mRNA in sensory neurons and also impaired long-term facilitation of sensory-to-motor synapses by repetitive 5-HT treatments. These results provide evidence for a critical role of the posttranscriptional modification of ApC/EBP mRNA during the consolidation of synaptic plasticity.
- Subjects :
- Untranslated region
Neural facilitation
Biology
Models, Biological
Synaptic Transmission
Genes, Reporter
Aplysia
Animals
Humans
Heterogeneous Nuclear Ribonucleoprotein D0
RNA, Messenger
Cloning, Molecular
Heterogeneous-Nuclear Ribonucleoprotein D
RNA Processing, Post-Transcriptional
3' Untranslated Regions
In Situ Hybridization
Messenger RNA
Reporter gene
Multidisciplinary
Neuronal Plasticity
Ccaat-enhancer-binding proteins
Models, Genetic
Biological Sciences
biology.organism_classification
Molecular biology
HEK293 Cells
Gene Expression Regulation
Synaptic plasticity
CCAAT-Enhancer-Binding Proteins
Immediate early gene
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 109
- Issue :
- 38
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....39d7560209a66e903b25986336455e50