Back to Search
Start Over
A single dual-emissive nanofluorophore test paper for highly sensitive colorimetry-based quantification of blood glucose
- Source :
- Biosensors and Bioelectronics. 86:530-535
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Fluorescent test papers are promising for the wide applications in the assays of diagnosis, environments and foods, but unlike classical dye-absorption-based pH test paper, they are usually limited in the qualitative yes/no type of detection by fluorescent brightness, and the colorimetry-based quantification remains a challenging task. Here, we report a single dual-emissive nanofluorophore probe to achieve the consecutive color variations from blue to red for the quantification of blood glucose on its as-prepared test papers. Red quantum dots were embedded into silica nanoparticles as a stable internal standard emission, and blue carbon dots (CDs) were further covalently linked onto the surface of silica, in which the ratiometric fluorescence intensity of blue to red is controlled at 5:1. While the oxidation of glucose induced the formation of Fe(3+) ions, the blue emission of CDs was thus quenched by the electron transfer from CDs to Fe(3+), displaying a serial of consecutive color variations from blue to red with the dosage of glucose. The high-quality test papers printed by the probe ink exhibited a dosage-sensitive allochromatic capability with the clear differentiations of ~5, 7, 9, 11mM glucose in human serum (normal: 3-8mM). The blood glucose determined by the test paper was almost in accordance with that measured by a standard glucometer. The method reported here opens a window to the wide applications of fluorescent test paper in biological assays.
- Subjects :
- Blood Glucose
Paper
Brightness
Silicon dioxide
Biomedical Engineering
Biophysics
Analytical chemistry
Biosensing Techniques
02 engineering and technology
010402 general chemistry
01 natural sciences
chemistry.chemical_compound
Electron transfer
Limit of Detection
Quantum Dots
Cadmium Compounds
Electrochemistry
Humans
Colorimetry
Fluorescent Dyes
Detection limit
Chemistry
General Medicine
Silicon Dioxide
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
Spectrometry, Fluorescence
Covalent bond
Quantum dot
Tellurium
0210 nano-technology
Biotechnology
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....39d37868146c42841427707ca580093d