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Differentiation of fetal sertoli cells in the adult testis
- Source :
- Reproduction
- Publication Year :
- 2021
-
Abstract
- Sertoli cells proliferate and construct seminiferous tubules during fetal life, then undergo differentiation and maturation in the prepubertal testes. In the adult testes, mature Sertoli cells maintain spermatogonia and support spermatogenesis during the entire lifetime. Although Sertoli-like cells have been derived from iPS cells, they tend to remain immature. To investigate whether Sertoli cells can spontaneously acquire the ability to support spermatogenesis when transferred into the adult testis, we transplanted mouse fetal testicular cells into a Sertoli-depleted adult testis. We found that donor E12.5, E14.5 and E16.5 Sertoli cells colonized adult seminiferous tubules and supported host spermatogenesis 2 months after transplantation, demonstrating that immature fetal Sertoli cells can undergo sufficient maturation in the adult testis to become functional. This technique will be useful to analyze the developmental process of Sertoli cell maturation and to investigate the potential of iPS-derived Sertoli cells to colonize, undergo maturation, and support spermatogenesis within the testis environment.
- Subjects :
- Male
endocrine system
Embryology
Biology
Article
Andrology
Mice
Endocrinology
Fetus
Pregnancy
Testis
medicine
Animals
Sexual Maturation
Induced pluripotent stem cell
Spermatogenesis
Sertoli Cells
urogenital system
Obstetrics and Gynecology
Cell Differentiation
Cell Biology
Seminiferous Tubules
Sertoli cell
Transplantation
medicine.anatomical_structure
Reproductive Medicine
Female
Subjects
Details
- ISSN :
- 17417899
- Volume :
- 162
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Reproduction (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....39a3c5a17d1f417e6c7d3c6cec7be783