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Characterization of lectin isolated from Momordica charantia seed as a B cell activator
- Source :
- Immunology letters. 121(2):148-156
- Publication Year :
- 2008
- Publisher :
- Elsevier Science, 2008.
-
Abstract
- Lectin isolated from the seeds of Momordica charantia (MCL) is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes (p < 0.05) in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate (FITC)-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin (Ig) was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants (p < 0.01). Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL.
- Subjects :
- Momordica charantia
T-Lymphocytes
Immunology
Naive B cell
B-Lymphocyte Subsets
Dose-Response Relationship, Immunologic
Immunoglobulins
Biology
Lymphocyte Activation
Mice
immune system diseases
hemic and lymphatic diseases
medicine
Splenocyte
Immunology and Allergy
Animals
Immunologic Factors
neoplasms
B cell
Cell Proliferation
chemistry.chemical_classification
B-Lymphocytes
Mice, Inbred BALB C
Momordica
Activator (genetics)
Lectin
Galactose
Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice
biology.organism_classification
Molecular biology
medicine.anatomical_structure
Biochemistry
chemistry
Seeds
biology.protein
B7-2 Antigen
Plant Lectins
Cell activation
Glycoprotein
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 01652478
- Volume :
- 121
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Immunology letters
- Accession number :
- edsair.doi.dedup.....399c45c9e67d75c4e6cf291147cb4158