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Antibodies targeting linear determinants of the envelope protein protect mice against West Nile virus
- Source :
- The Journal of infectious diseases. 196(12)
- Publication Year :
- 2008
-
Abstract
- The flavivirus envelope (E) protein mediates cellular attachment and fusion with host cell membranes and is recognized by virus-neutralizing antibodies. We raised antibodies against a broad range of epitopes by immunizing a horse with recombinant West Nile virus (WNV) E protein. To define epitopes recognized by protective antibodies, we selected, by affinity chromatography, immunoglobulins against immobilized linear peptides derived from parts of the E protein. Immunoglobulins binding 9 different peptides from domains I, II, and III of the E protein neutralized WNV in vitro. This indicates that multiple protective epitopes can be found in the E protein. Immunoglobulins recognizing 3 peptides derived from domains I and II of E protein protected mice against a lethal challenge with WNV. These immunoglobulins recognized the E proteins of related flaviviruses, demonstrating that antibodies targeting specific E protein epitopes could be developed for prevention and treatment of multiple flavivirus infections.
- Subjects :
- viruses
Molecular Sequence Data
Antibody Affinity
Fluorescent Antibody Technique
Immunoglobulins
Antibodies, Viral
Epitope
Virus
law.invention
Flaviviridae
Epitopes
Mice
Affinity chromatography
Viral Envelope Proteins
law
Chlorocebus aethiops
Immunology and Allergy
Animals
Flavivirus Infections
Amino Acid Sequence
Horses
Vero Cells
Mice, Inbred C3H
biology
Immunization, Passive
virus diseases
biology.organism_classification
Virology
Peptide Fragments
Flavivirus
Infectious Diseases
biology.protein
Recombinant DNA
Drosophila
Female
Antibody
West Nile virus
West Nile Fever
Subjects
Details
- ISSN :
- 00221899
- Volume :
- 196
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The Journal of infectious diseases
- Accession number :
- edsair.doi.dedup.....3990b0631031eb5372ef115ce8da7028