Back to Search
Start Over
Impact of Antibiotic-Resistant Bacteria on Immune Activation and Clostridioides difficile Infection in the Mouse Intestine
- Source :
- Infect Immun
- Publication Year :
- 2019
-
Abstract
- Antibiotic treatment of patients undergoing complex medical treatments can deplete commensal bacterial strains from the intestinal microbiota, thereby reducing colonization resistance against a wide range of antibiotic-resistant pathogens. Loss of colonization resistance can lead to marked expansion of vancomycin-resistant Enterococcus faecium (VRE), Klebsiella pneumoniae, and Escherichia coli in the intestinal lumen, predisposing patients to bloodstream invasion and sepsis. The impact of intestinal domination by these antibiotic-resistant pathogens on mucosal immune defenses and epithelial and mucin-mediated barrier integrity is unclear. We used a mouse model to study the impact of intestinal domination by antibiotic-resistant bacterial species and strains on the colonic mucosa. Intestinal colonization with K. pneumoniae, Proteus mirabilis, or Enterobacter cloacae promoted greater recruitment of neutrophils to the colonic mucosa. To test the hypothesis that the residual microbiota influences the severity of colitis caused by infection with Clostridioides difficile, we coinfected mice that were colonized with ampicillin-resistant bacteria with a virulent strain of C. difficile and monitored colonization and pathogenesis. Despite the compositional differences in the gut microbiota, the severity of C. difficile infection (CDI) and mortality did not differ significantly between mice colonized with different ampicillin-resistant bacterial species. Our results suggest that the virulence mechanisms enabling CDI and epithelial destruction outweigh the relatively minor impact of less-virulent antibiotic-resistant intestinal bacteria on the outcome of CDI.
- Subjects :
- medicine.drug_class
Immunology
Antibiotics
Virulence
Colonisation resistance
Gut flora
Microbiology
Mice
Antibiotic resistance
Drug Resistance, Bacterial
Enterobacter cloacae
medicine
Animals
Colonization
Colitis
Proteus mirabilis
biology
Enterobacteriaceae Infections
Bacterial Infections
biology.organism_classification
medicine.disease
Survival Analysis
Anti-Bacterial Agents
Disease Models, Animal
Klebsiella pneumoniae
Infectious Diseases
Clostridium Infections
Microbial Interactions
Parasitology
Enterococcus faecium
Subjects
Details
- ISSN :
- 10985522
- Volume :
- 88
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Infection and immunity
- Accession number :
- edsair.doi.dedup.....398e7fa37d04b9992e9434c795b02793