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Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize
- Source :
- The Plant Journal. 109:980-991
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map-based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities for ECs. We demonstrated that ZmSAUR15, which encodes a small auxin-upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole-3-acetic acid biosynthesis and cell division in immature embryo-derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
- Subjects :
- Cloning
Cell division
Arabidopsis Proteins
Effector
Transgene
Arabidopsis
Intracellular Signaling Peptides and Proteins
Genetic Variation
Embryo
Cell Biology
Plant Science
Biology
Plants, Genetically Modified
Zea mays
Embryonic stem cell
Cell biology
Phenotype
Inbred strain
Gene Expression Regulation, Plant
Callus
Genetics
Promoter Regions, Genetic
Cell Division
Plant Proteins
Subjects
Details
- ISSN :
- 1365313X and 09607412
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- The Plant Journal
- Accession number :
- edsair.doi.dedup.....39845bf67cfcbac3226f9dc5c33e2cf6