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Fine mapping of the Rsv1-h gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus
- Source :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 129(11)
- Publication Year :
- 2016
-
Abstract
- The Rsv1 - h gene in cultivar Suweon 97, which confers resistance to SMVs, was mapped to a 97.5-kb location (29,815,195–29,912,667 bp on chromosome 13) in the Rsv1 locus, thereby providing additional insights into the molecular nature underlying variations in resistance alleles in this particular locus. Soybean mosaic virus (SMV) is a well-known devastating pathogen of soybean (Glycine max (L.) Merrill.) causing significant yield losses and seed quality deterioration. A single dominant allele, Rsv1-h, which confers resistance to multiple SMV strains, was previously reported in the cultivar Suweon 97, but its exact location is unknown. In the present study, Suweon 97 was crossed with a SMV-sensitive cultivar, Williams 82. Inoculating 267 F 2 individuals with two Chinese SMV strains (SC6-N and SC7-N) demonstrated that one single dominant gene confers SMV resistance. Another 1,150 F 2 individuals were then screened for two simple sequence repeat (SSR) markers (BARCSOYSSR_13_1103 and BARCSOYSSR_13_1187) that flank the Rsv1 locus. Seventy-four recombinants were identified and 20 additional polymorphic SSR markers within the Rsv1 region were then employed in genotyping these recombinants. F 2:3 and F 3:4 recombinant lines were also inoculated with SC6-N and SC7-N to determine their phenotypes. The final data revealed that in Suweon 97, the Rsv1-h gene that confers resistance to SC6-N and SC7-N was flanked by BARCSOYSSR_13_1114 and BARCSOYSSR_13_1115, two markers that delimit a 97.5-kb region in the reference Williams 82 genome. In such region, eight genes were present, of which two, Glyma13g184800 and Glyma13g184900, encode the characteristic CC-NBS-LRR type of resistance gene and were considered potential candidates for Rsv1-h.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetic Markers
DNA, Plant
Potyvirus
Soybean mosaic virus
Locus (genetics)
Genes, Plant
01 natural sciences
Genome
03 medical and health sciences
Genetics
Allele
Genotyping
Gene
Crosses, Genetic
Disease Resistance
Genes, Dominant
Plant Diseases
biology
food and beverages
General Medicine
biology.organism_classification
030104 developmental biology
Phenotype
Genetic marker
Soybeans
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Microsatellite Repeats
Subjects
Details
- ISSN :
- 14322242
- Volume :
- 129
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
- Accession number :
- edsair.doi.dedup.....396d3fbaff37fd6e5b5369b45b4479e5