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Prognostic value of quantitative measurement of EGFR mutation using peptide nucleic acid clamping in advanced EGFR mutant non‐small cell lung cancer patients
- Source :
- Thoracic Cancer, Thoracic Cancer, Vol 10, Iss 7, Pp 1561-1566 (2019)
- Publication Year :
- 2019
- Publisher :
- John Wiley & Sons Australia, Ltd, 2019.
-
Abstract
- Background The presence of EGFR mutation in patients with advanced non-small cell lung cancer (NSCLC) plays an important role in determining the appropriate treatment, response, and survival. Therefore, this study attempted to predict the prognosis of NSCLC patients using data from quantitative mutation measurements. Methods The data of patients with advanced NSCLC who underwent EGFR mutation testing using the peptide nucleic acid (PNA) mediated clamping method at the Pusan National University Hospital from October 2015 to December 2017 were retrospectively analyzed. The efficiency of PNA clamping was determined by measuring the threshold cycle (Ct ) value. The ΔCt -1 value (standard Ct value minus sample Ct value) was calculated to quantify EGFR mutation. Results During the study period, 71 patients were treated with EGFR-tyrosine kinase inhibitors. The cutoff point for the ΔCt -1 value derived from the receiver operating characteristic curve was 5.32. A survival benefit was observed in the group with an ΔCt -1 value > 5.32 or with a common EGFR mutation type compared to the group with an ΔCt -1 value Conclusion EGFR mutation testing using PNA clamping may predict patient survival, especially in patients with common EGFR mutations, such as exon 19 deletion or L858R. A higher ΔCt -1 value correlates with better survival.
- Subjects :
- 0301 basic medicine
Oncology
Male
Peptide Nucleic Acids
Lung Neoplasms
Mutant
Exon
chemistry.chemical_compound
0302 clinical medicine
Carcinoma, Non-Small-Cell Lung
Epidermal growth factor receptor
Sequence Deletion
Peptide nucleic acid
biology
General Medicine
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Prognosis
ErbB Receptors
Treatment Outcome
030220 oncology & carcinogenesis
Mutation (genetic algorithm)
peptide nucleic acid
Original Article
Female
Pulmonary and Respiratory Medicine
medicine.medical_specialty
molecular targeted therapy
Peptide Nucleic Acid Clamping
lcsh:RC254-282
03 medical and health sciences
Internal medicine
medicine
Biomarkers, Tumor
Humans
Genetic Testing
Lung cancer
Protein Kinase Inhibitors
Retrospective Studies
Receiver operating characteristic
business.industry
Original Articles
medicine.disease
Survival Analysis
lung cancer
030104 developmental biology
chemistry
Amino Acid Substitution
ROC Curve
Mutation
biology.protein
business
Subjects
Details
- Language :
- English
- ISSN :
- 17597714 and 17597706
- Volume :
- 10
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Thoracic Cancer
- Accession number :
- edsair.doi.dedup.....3958a4af4509d57b738a57ba178fec65