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[Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection]
- Source :
- Zhonghua zhong liu za zhi [Chinese journal of oncology]. 19(3)
- Publication Year :
- 1997
-
Abstract
- AIM: To demonstrate the relationship between H-ras oncogene and hepatocellular carcinoma (HCC) metastasis. METHODS: Activated H-ras oncogene was transfected into SMMC 7721, a cell line derived from human HCC, by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expression of collagenase IV (cIVase) and epidermal growth factor receptor (EGFR). RESULTS: The abilities of H-ras-transfected cell clones in adhesion to laminin (LN) or fibronectin (FN), migration, cIVase secretion increased markedly, and the expression of EGFR elevated moderately. More importantly, these alterations were consistent positively with the expression of p21, the protein product of H-ras oncogene. CONCLUSION: H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro to raise its metastatic potential.
- Subjects :
- Carcinoma, Hepatocellular
Cell
In Vitro Techniques
Transfection
Proto-Oncogene Proteins p21(ras)
Laminin
Cell Movement
Predictive Value of Tests
medicine
Cell Adhesion
Tumor Cells, Cultured
Humans
Epidermal growth factor receptor
Neoplasm Metastasis
Cell adhesion
neoplasms
Oncogene
biology
Liver Neoplasms
Gastroenterology
General Medicine
Original Articles
digestive system diseases
Fibronectin
Gene Expression Regulation, Neoplastic
ErbB Receptors
medicine.anatomical_structure
Phenotype
Genes, ras
Matrix Metalloproteinase 9
Cell culture
Cell Migration Inhibition
biology.protein
Cancer research
Matrix Metalloproteinase 2
Subjects
Details
- ISSN :
- 02533766
- Volume :
- 19
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Zhonghua zhong liu za zhi [Chinese journal of oncology]
- Accession number :
- edsair.doi.dedup.....394f5b5a697d6655c3b919da453c0967