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Trichomonas vaginalis Detection in Urogenital Specimens from Symptomatic and Asymptomatic Men and Women by Use of the cobas TV/MG Test
- Source :
- Journal of clinical microbiology, vol 59, iss 10, Journal of Clinical Microbiology
- Publication Year :
- 2021
- Publisher :
- eScholarship, University of California, 2021.
-
Abstract
- Trichomonas vaginalis is a prevalent sexually transmitted infection (STI). Diagnosis has historically relied on either microscopic analysis or culture, the latter being the previous gold standard. However, these tests are not readily available for male diagnosis, generally only perform well for symptomatic women, and are not as sensitive as nucleic acid amplification tests (NAATs). Men are largely asymptomatic but carry the organism and transmit to their sexual partners. This multicenter, prospective study evaluated the performance of the cobas T. vaginalis/Mycoplasma genitalium (TV/MG) assay for detection of T. vaginalis DNA compared with patient infection status (PIS) defined by a combination of commercially available NAATs and culture using urogenital specimens. A total of 2,064 subjects (984 men and 1,080 women, 940 [45.5%] symptomatic, 1,124 [54.5%] asymptomatic) were evaluable. In women, sensitivity ranged from 99.4% (95% confidence interval [CI] 96.8 to 99.9%) using vaginal samples to 94.7% (95% CI 90.2 to 97.2%) in PreservCyt samples. Specificity ranged from 98.9 to 96.8% (95% CI 95.4 to 97.8%). In men, the cobas TV/MG assay was 100% sensitive for the detection of T. vaginalis in both male urine samples and meatal swabs, with specificity of 98.4% in urine samples and 92.5% in meatal swabs. The cobas TV/MG is a suitable diagnostic test for the detection of T. vaginalis, which could support public health efforts toward infection control and complement existing STI programs.
- Subjects :
- Male
Urine
molecular methods
medicine.disease_cause
Medical and Health Sciences
0302 clinical medicine
Prevalence
Medicine
Infection control
NAAT
030212 general & internal medicine
Prospective Studies
Prospective cohort study
screening and diagnosis
biology
Biological Sciences
Detection
Infectious Diseases
Vagina
Female
medicine.symptom
0305 other medical science
Trichomonas Vaginitis
Infection
4.2 Evaluation of markers and technologies
Microbiology (medical)
Urologic Diseases
medicine.medical_specialty
Sexually Transmitted Diseases
Asymptomatic
Sensitivity and Specificity
Microbiology
03 medical and health sciences
parasitic diseases
Trichomonas vaginalis
Humans
urogenital
Gynecology
030505 public health
Agricultural and Veterinary Sciences
business.industry
Genitourinary system
Gold standard (test)
biology.organism_classification
Good Health and Well Being
Sexually Transmitted Infections
Parasitology
business
Mycoplasma genitalium
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Journal of clinical microbiology, vol 59, iss 10, Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....39381a5d4f2aeef761defdb47590e674