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Permeabilization of the nuclear envelope following nanosecond pulsed electric field exposure
- Source :
- Biochemical and biophysical research communications. 470(1)
- Publication Year :
- 2015
-
Abstract
- Permeabilization of cell membranes occurs upon exposure to a threshold absorbed dose (AD) of nanosecond pulsed electric fields (nsPEF). The ultimate, physiological bioeffect of this exposure depends on the type of cultured cell and environment, indicating that cell-specific pathways and structures are stimulated. Here we investigate 10 and 600 ns duration PEF effects on Chinese hamster ovary (CHO) cell nuclei, where our hypothesis is that pulse disruption of the nuclear envelope membrane leads to observed cell death and decreased viability 24 h post-exposure. To observe short-term responses to nsPEF exposure, CHO cells have been stably transfected with two fluorescently-labeled proteins known to be sequestered for cellular chromosomal function within the nucleus - histone-2b (H2B) and proliferating cell nuclear antigen (PCNA). H2B remains associated with chromatin after nsPEF exposure, whereas PCNA leaks out of nuclei permeabilized by a threshold AD of 10 and 600 ns PEF. A downturn in 24 h viability, measured by MTT assay, is observed at the number of pulses required to induce permeabilization of the nucleus.
- Subjects :
- 0301 basic medicine
Programmed cell death
Cell Membrane Permeability
Cell Survival
Nuclear Envelope
Cell
Biophysics
Apoptosis
CHO Cells
Biology
Radiation Dosage
Biochemistry
03 medical and health sciences
Cricetulus
Electromagnetic Fields
Cricetinae
medicine
Animals
MTT assay
Molecular Biology
Chinese hamster ovary cell
Electroporation
Dose-Response Relationship, Radiation
Cell Biology
Proliferating cell nuclear antigen
Cell biology
030104 developmental biology
medicine.anatomical_structure
biology.protein
Nucleus
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 470
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....3937a8bb588feaf1f6fa3c7cf2c1c40a