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T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides
- Source :
- Journal of the American Academy of Dermatology. 73(2)
- Publication Year :
- 2014
-
Abstract
- Background T-cell receptor (TCR) clonality assessment is a principal diagnostic test in the management of mycosis fungoides (MF). However, current polymerase chain reaction–based methods may produce ambiguous results, often because of low abundance of clonal T lymphocytes, resulting in weak clonal peaks that cannot be size-resolved by contemporary capillary electrophoresis (CE). Objective We sought to determine if next-generation sequencing (NGS)-based detection has increased sensitivity for T-cell clonality over CE-based detection in MF. Methods Clonality was determined by an NGS-based method in which the TCR-γ variable region was polymerase chain reaction amplified and the products sequenced to establish the identity of rearranged variable and joining regions. Results Of the 35 MF cases tested, 29 (85%) showed a clonal T-cell rearrangement by NGS, compared with 15 (44%) by standard CE detection. Three patients with MF had follow-up testing that showed identical, clonal TCR sequences in subsequent skin biopsy specimens. Limitations Clonal T-cell populations have been described in benign conditions; evidence of clonality alone, by any method, is not sufficient for diagnosis. Conclusion TCR clonality assessment by NGS has superior sensitivity compared with CE-based detection. Further, NGS enables tracking of specific clones across multiple time points for more accurate identification of recurrent MF.
- Subjects :
- Adult
Electrophoresis
Male
Skin Neoplasms
Databases, Factual
T cell
Molecular Sequence Data
Receptors, Antigen, T-Cell
Dermatology
Biology
T-cell receptor rearrangement
Polymerase Chain Reaction
DNA sequencing
law.invention
molecular diagnostics
Mycosis Fungoides
law
medicine
Humans
Genetic Predisposition to Disease
cutaneous T-cell lymphoma
Cloning, Molecular
Polymerase chain reaction
Aged
Retrospective Studies
Mycosis fungoides
T-cell clonality
medicine.diagnostic_test
Cutaneous T-cell lymphoma
T-cell receptor
DNA, Neoplasm
Middle Aged
medicine.disease
Molecular diagnostics
Prognosis
Molecular biology
medicine.anatomical_structure
Skin biopsy
next-generation sequencing
Female
Neoplasm Recurrence, Local
Subjects
Details
- ISSN :
- 10976787
- Volume :
- 73
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of the American Academy of Dermatology
- Accession number :
- edsair.doi.dedup.....392a9dfae38bd468e46f0a10abc39670