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Dedifferentiation of MCF-7 Breast Cancer Continuous Cell Line, Development of Breast Cancer Stem Cells (BCSCs) Enriched Culture and Biomarker Analysis
- Source :
- Indonesian Biomedical Journal, Vol 12, Iss 2, Pp 115-23 (2020)
- Publication Year :
- 2020
- Publisher :
- Secretariat of The Indonesian Biomedical Journal, 2020.
-
Abstract
- BACKGROUND: Cancer stem cells (CSCs) eradication might serve as a robust approach for cancer eradication. MCF-7 as breast cancer continuous cell line is known to contain breast CSCs (BCSCs) for its capability to maintain its original tumor population. CSCs enriched culture is a fundamental tool for CSCs targeted therapy development. Effective and unsophisticated CSCs dedifferentiation protocol for producing CSCs enriched culture is needed.METHODS: MCF-7 cells were cultured initially in Dulbecco's Modified Eagle Medium (DMEM) low glucose medium then changed to DMEM:F12. Serum starvation was performed during each medium refreshment gradually with fetal bovine serum (FBS) concentration of 10%, 5%, 2.5% until reaching 1% FBS concentration. Stable MCF-7 culture was then adapted to serum free culture system, containing DMEM:F12, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and B27 supplement as dedifferentiation protocol for 18 days. Cluster of differentiation (CD)44 and CD24 double staining immunocytochemistry was performed to evaluate cell stemness.RESULTS: The population of cells expressing BCSCs markers (CD44+/CD24low) in non-adherent single cells subpopulation was significantly increased after the dedifferentiation procedure (70.39%) compared to control groups (0.71%) (p
- Subjects :
- lcsh:R5-920
education.field_of_study
biology
CD44
Population
Medicine (miscellaneous)
Cancer
medicine.disease
General Biochemistry, Genetics and Molecular Biology
Breast cancer
MCF-7
Cancer stem cell
Cell culture
Cancer research
biology.protein
medicine
Stem cell
lcsh:Medicine (General)
skin and connective tissue diseases
education
Subjects
Details
- ISSN :
- 23559179 and 20853297
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- The Indonesian Biomedical Journal
- Accession number :
- edsair.doi.dedup.....39269279fb0195cd84556740a1afeaf0