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3-O-sulfated heparan sulfate interactors target synaptic adhesion molecules from neonatal mouse brain and inhibit neural activity and synaptogenesis in vitro
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports, Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.19114. ⟨10.1038/s41598-020-76030-4⟩, Scientific Reports, 10, 1, Scientific Reports, 2020, 10 (1), pp.19114. ⟨10.1038/s41598-020-76030-4⟩, Scientific Reports, 10
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Heparan sulfate (HS) chains, covalently linked to heparan sulfate proteoglycans (HSPG), promote synaptic development and functions by connecting various synaptic adhesion proteins (AP). HS binding to AP could vary according to modifications of HS chains by different sulfotransferases. 3-O-sulfotransferases (Hs3sts) produce rare 3-O-sulfated HSs (3S-HSs), of poorly known functions in the nervous system. Here, we showed that a peptide known to block herpes simplex virus by interfering with 3S-HSs in vitro and in vivo (i.e. G2 peptide), specifically inhibited neural activity, reduced evoked glutamate release, and impaired synaptic assembly in hippocampal cell cultures. A role for 3S-HSs in promoting synaptic assembly and neural activity is consistent with the synaptic interactome of G2 peptide, and with the detection of Hs3sts and their products in synapses of cultured neurons and in synaptosomes prepared from developing brains. Our study suggests that 3S-HSs acting as receptors for herpesviruses might be important regulators of neuronal and synaptic development in vertebrates.
- Subjects :
- Nervous system
Neurogenesis
[SDV]Life Sciences [q-bio]
Synaptogenesis
lcsh:Medicine
Peptide
Hippocampus
Interactome
Article
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Receptor
lcsh:Science
Cells, Cultured
030304 developmental biology
Neurons
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
integumentary system
Cell adhesion molecule
lcsh:R
Glutamate receptor
Development of the nervous system
Heparan sulfate
Synaptic development
3. Good health
Cell biology
[SDV] Life Sciences [q-bio]
medicine.anatomical_structure
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
chemistry
Synapses
lcsh:Q
Heparitin Sulfate
Sulfotransferases
Heparan Sulfate Proteoglycans
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3920d7ce994c5c36827e950b1b3debba