Back to Search
Start Over
Directional topography gradients drive optimum alignment and differentiation of human myoblasts
- Source :
- Journal of tissue engineering and regenerative medicine. Wiley, Journal of Tissue Engineering and Regenerative Medicine
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- Tissue engineering of skeletal muscle aims to replicate the parallel alignment of myotubes on the native tissue. Directional topography gradients allow the study of the influence of topography on cellular orientation, proliferation, and differentiation, resulting in yield cues and clues to develop a proper in vitro environment for muscle tissue engineering. In this study, we used a polydimethylsiloxane-based substrate containing an aligned topography gradient with sinusoidal features ranging from wavelength (lambda) = 1,520 nm and amplitude (A) =176 nm to lambda = 9,934 nm and A = 2,168 nm. With this topography gradient, we evaluated the effect of topography on human myoblasts distribution, dominant orientation, cell area, nuclei coverage, cell area per number of nuclei, and nuclei area of myotubes. We showed that human myoblasts aligned and differentiated irrespective of the topography section. In addition, aligned human myotubes showed functionality and maturity by contracting spontaneously and nuclei peripheral organization resembling natural myotubes.
- Subjects :
- Adult
Male
Muscle tissue
Materials science
Myoblasts, Skeletal
Muscle Fibers, Skeletal
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
ORGANIZATION
02 engineering and technology
polydimethylsiloxane (PDMS)
Biomaterials
03 medical and health sciences
Tissue engineering
SATELLITE CELLS
medicine
Humans
Myocyte
Research Articles
030304 developmental biology
0303 health sciences
Tissue Scaffolds
Myogenesis
myoblasts
Skeletal muscle
Cell Differentiation
Middle Aged
MUSCLE
020601 biomedical engineering
MODEL
topography gradient
medicine.anatomical_structure
myotubes
SCREENING PLATFORM
tissue engineering
Native tissue
PATTERNS
Biophysics
Female
ORIENTATION
Research Article
Subjects
Details
- ISSN :
- 19327005 and 19326254
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Tissue Engineering and Regenerative Medicine
- Accession number :
- edsair.doi.dedup.....38f727d8b24ed89661429136e480bfc7