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Unusual branch point selection in processing of human growth hormone pre-mRNA
- Source :
- Europe PubMed Central
-
Abstract
- Intron A of the human growth hormone gene does not contain an A residue within 56 nucleotides preceding the 3' splice site. The analysis of the excised intron lariat revealed a C residue 28 nucleotides upstream from the 3' splice site as the major branch acceptor nucleotide. Two additional minor branched nucleotides were identified as U residues at positions -22 and -36. An adenosine substitution at position -22 results in lariat formation solely to this nucleotide. Therefore, C and U residues can function efficiently as natural branch acceptors, but an A residue is preferred if available in the proper region. In addition, the data strongly reinforce the importance of the distance constraint for lariat formation. To explain selection of the branch acceptor nucleotide, potential base-pairing interactions of branch point sequences with the U2 RNA are discussed.
- Subjects :
- RNA Splicing
Molecular Sequence Data
Biology
Residue (chemistry)
RNA Precursors
Humans
Nucleotide
Molecular Biology
chemistry.chemical_classification
Messenger RNA
Base Sequence
Intron
RNA
Nucleic Acid Hybridization
Cell Biology
Ribonucleoproteins, Small Nuclear
Introns
Biochemistry
chemistry
Ribonucleoproteins
Growth Hormone
RNA splicing
Nucleic acid
Precursor mRNA
HeLa Cells
Research Article
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....38f2fe5dedf3ba675506d79f1a7b6980