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Cell cycle dependent changes in the plasma membrane organization of mammalian cells
- Source :
- Biochimica et biophysica acta. Biomembranes. 1859(3)
- Publication Year :
- 2016
-
Abstract
- Lipid membranes are major structural elements of all eukaryotic and prokaryotic organisms. Although many aspects of their biology have been studied extensively, their dynamics and lateral heterogeneity are still not fully understood. Recently, we observed a cell-to-cell variability in the plasma membrane organization of CHO-K1 cells (Schwarzer et al., 2014). We surmised that cell cycle dependent changes of the individual cells from our unsynchronized cell population account for this phenomenon. In the present study, this hypothesis was tested. To this aim, CHO-K1 cells were arrested in different cell cycle phases by chemical treatments, and the order of their plasma membranes was determined by various fluorescent lipid analogues using fluorescence lifetime imaging microscopy. Our experiments exhibit significant differences in the membrane order of cells arrested in the G2/M or S phase compared to control cells. Our single-cell analysis also enabled the specific selection of mitotic cells, which displayed a significant increase of the membrane order compared to the control. In addition, the lipid raft marker GPImYFP was used to study the lateral organization of cell cycle arrested cells as well as mitotic cells and freely cycling samples. Again, significant differences were found between control and arrested cells and even more pronounced between control and mitotic cells. Our data demonstrate a direct correlation between cell cycle progression and plasma membrane organization, underlining that cell-to-cell heterogeneities of membrane properties have to be taken into account in cellular studies especially at the single-cell level.
- Subjects :
- 0301 basic medicine
M Phase Cell Cycle Checkpoints
Cell
Population
Biophysics
Medizin
CHO Cells
Biology
Biochemistry
03 medical and health sciences
Cricetulus
Membrane Microdomains
Cricetinae
medicine
Animals
education
Lipid raft
Mitosis
education.field_of_study
Plasma membrane organization
030102 biochemistry & molecular biology
G2 Phase Cell Cycle Checkpoints
Cell Membrane
Membrane Proteins
Cell Biology
Cell cycle
Cell biology
030104 developmental biology
medicine.anatomical_structure
Microscopy, Fluorescence
S Phase Cell Cycle Checkpoints
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 1859
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta. Biomembranes
- Accession number :
- edsair.doi.dedup.....38e352e1ab69742832ebf0c60ad54754