Back to Search
Start Over
Sister Chromatid Exchanges in Cultured Amniocytes Exposed to Diagnostic Ultrasound in Vitro
- Source :
- Acta Radiologica. 35:58-61
- Publication Year :
- 1994
- Publisher :
- SAGE Publications, 1994.
-
Abstract
- An in vitro system has been set up to study the possible genetic effects of diagnostic ultrasound (US) on the developing fetus. Amniotic cells were cultured by the in situ technique. Well established clones were exposed to US for various lengths of time using a linear array transducer within a sound transparent apparatus. Cells were then grown in the presence of 5-bromo-2'-deoxyuridine and processed for scoring the number of sister chromatid exchanges (SCE) as the cytogenetic endpoint. There was no linear relationship between the SCE frequency and the duration of US exposure. Variance analysis showed that only interindividual variability was a significant component of total variation. Neither the main effect of treatment nor the interaction effect were statistically significant. The data suggest that US delivered from a diagnostic unit to actively growing cultured fetal cells in a system closely mimicking the conditions of US exposure during amniocentesis does not induce SCE.
- Subjects :
- Adult
Pathology
medicine.medical_specialty
Diagnostic ultrasound
Ultrasonography, Prenatal
Andrology
Pregnancy
medicine
Humans
Sister chromatids
Ultrasonics
Radiology, Nuclear Medicine and imaging
Cells, Cultured
Analysis of Variance
Fetus
Radiological and Ultrasound Technology
medicine.diagnostic_test
business.industry
Cell Cycle
General Medicine
Amniotic Fluid
In vitro
Linear relationship
Cell culture
Amniocentesis
Female
Chromatid
business
Sister Chromatid Exchange
Subjects
Details
- ISSN :
- 16000455 and 02841851
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Acta Radiologica
- Accession number :
- edsair.doi.dedup.....38d3fe79f326fd14253148c08bdd1a43