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Rifampicin loaded in alginate/chitosan nanoparticles as a promising pulmonary carrier against Staphylococcus aureus
- Source :
- Drug Delivery and Translational Research. 10:1403-1417
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- This study aims to explore the antimicrobial activity of rifampicin (RIF) and ascorbic acid (ASC) co-loaded into alginate (ALG)/chitosan (CS) nanoparticles (RIF/ASC NPs) and tested for their antibacterial activity against several strains of methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Also, the present research focused on exploring the possible antibacterial mechanism of action of these RIF/ASC NPs, which demonstrated a significant biocide activity against the S. aureus strains with minimum inhibitory concentrations (MIC) between 2- and 8-fold lower than those one exhibited with the free antibiotic RIF. The proposed antimicrobial mechanism of action of the RIF/ASC NPs seems to be the result of collaborative effects between NPs and the RIF/ASC antibiotic combination. Moreover, results indicated that the functionalized RIF/ASC NP surface played a crucial role on the processes of NP adhesion into the bacterial surface, the alterations on the cell membrane integrity, and the cell uptake of the RIF/ASC antibiotic into bacteria. Further, the in vivo lung deposition pattern of empty NPs labeled (NPs-FITC) with isothiocyanate fluorescein in rats was investigated post intratracheal instillation of NPs. In summary, findings from this work show that our novel designed engineered RIF/ASC co-loaded NPs could be a suitable system for antibiotic lung administration with promising perspectives for effective treatments of pulmonary intracellular infections for those known antibiotics that are losing effectiveness due to antimicrobial resistance problems. Graphical Abstract.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Alginates
medicine.drug_class
Antibiotics
Pharmaceutical Science
02 engineering and technology
medicine.disease_cause
030226 pharmacology & pharmacy
Microbiology
Chitosan
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
polycyclic compounds
medicine
Animals
Lung
Chemistry
technology, industry, and agriculture
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
021001 nanoscience & nanotechnology
Ascorbic acid
Antimicrobial
Anti-Bacterial Agents
Rats
Nanoparticles
bacteria
Rifampin
0210 nano-technology
Antibacterial activity
Rifampicin
medicine.drug
Subjects
Details
- ISSN :
- 21903948 and 2190393X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Drug Delivery and Translational Research
- Accession number :
- edsair.doi.dedup.....38d0b4afe9b2613e1ad28915bcb00a08