Back to Search
Start Over
Induction of Fas and Fas Ligand Expression on Malignant Glioma Cells by Kupffer Cells, a Potential Pathway of Antiliver Metastases
- Source :
- Journal of Surgical Research. 101:44-51
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- Kupffer cells play an important role in controlling the growth and development of liver metastases. However, the pathway of Kupffer cells against tumor metastases is not clear. In the present study, we set up an experimental model to investigate the mechanisms on how Kupffer cells kill tumor cells which metastasize to the liver. Malignant glioma cells were cocultured with Kupffer cells or treated with culture medium collected from lipopolysaccharide (LPS)-activated Kupffer cells. The results showed that the interaction between Kupffer cells and malignant glioma cells significantly stimulated the generation of tumor necrosis factoralpha (TNFalpha). TNFalpha was mainly produced by Kupffer cells, as its level in culture medium obtained from LPS-treated Kupffer cells was not significantly different from that of malignant glioma cells treated with the same medium. Both Kupffer cells and LPS/Kupffer cell-conditioned supernatants induced expression of Fas and Fas ligand on malignant glioma cells. Subsequently a significant proportion of malignant glioma cells became apoptotic, as evidenced by positive staining of annexin V and propidium iodine and an increase in cellular DNA fragmentation. Therefore, this study supports a novel pathway of Kupffer cells against liver metastases, in which tumor cells were apoptotic via the Fas-Fas ligand system induced by TNFalpha released from Kupffer cells.
- Subjects :
- Pathology
medicine.medical_specialty
Fas Ligand Protein
Kupffer Cells
Liver cytology
medicine.medical_treatment
Apoptosis
Cell Communication
Biology
digestive system
Fas ligand
Flow cytometry
Glioma
medicine
Animals
fas Receptor
Membrane Glycoproteins
medicine.diagnostic_test
Liver Neoplasms
Kupffer cell
medicine.disease
Rats
medicine.anatomical_structure
Cytokine
Cancer research
Surgery
Tumor necrosis factor alpha
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Journal of Surgical Research
- Accession number :
- edsair.doi.dedup.....3887bf0d1693ddc669fe60c414f2dbd0