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Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
- Publication Year :
- 2017
-
Abstract
- Monocarboxylate transporter-1 (MCT-1) is a transmembrane transporter for monocarboxylates including lactate and pyruvate. Silencing Mct1 by its small interfering RNA (siRNA) suppressed the expression of marker genes for osteoblast differentiation, namely, Tnap, Runx2, and Sp7, induced by BMP-2 in mouse myoblastic C2C12 cells. Mct1 siRNA also suppressed alkaline phosphatase activity, as well as expressions of Tnap and Bglap mRNAs in mouse primary osteoblasts. On the other hand, Mct1 siRNA did not have effects on the Smad1/5 or ERK/JNK pathways in BMP-2-stimulated C2C12 cells, while it up-regulated the mRNA expression of p53 (Trp53) as well as nuclear accumulation of p53 in C2C12 cells in a BMP-2-independent manner. Suppression of osteoblastic differentiation by Mct1 siRNA in C2C12 cells was abolished by co-transfection of Trp53 siRNA. Together, these results suggest that MCT-1 functions as a positive regulator of osteoblast differentiation via suppression of p53.
- Subjects :
- 0301 basic medicine
Monocarboxylic Acid Transporters
Small interfering RNA
Cellular differentiation
Primary Cell Culture
lcsh:Medicine
Bone Morphogenetic Protein 2
Article
Cell Line
Myoblasts
03 medical and health sciences
Mice
medicine
Gene silencing
Animals
RNA, Small Interfering
lcsh:Science
Multidisciplinary
Osteoblasts
biology
Symporters
Chemistry
lcsh:R
Osteoblast
Cell Differentiation
musculoskeletal system
Recombinant Proteins
Cell biology
RUNX2
030104 developmental biology
medicine.anatomical_structure
Monocarboxylate transporter 1
Cell culture
Gene Knockdown Techniques
biology.protein
lcsh:Q
Tumor Suppressor Protein p53
C2C12
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....387f078c76f075294c2a5a5809137809