Back to Search
Start Over
In vitro modulatory effect of ω-3 polyunsaturated fatty acid (EPA and DHA) on phagocytosis and ROS production of goat neutrophils
- Source :
- Veterinary Immunology and Immunopathology. 131:79-85
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- An in vitro study was carried out to examine the influence of two fish-oil-derived long chain omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on goat polymorphonuclear leukocytes (PMN). Twelve Saanen healthy goats were used as blood donors. Neutrophils were isolated from blood and incubated with increasing concentration of EPA and DHA (25, 50, 100, 200muM). Control samples were incubated in the absence of omega-3 PUFAs. Phagocytosis was evaluated by fluorescein-labeled Escherichia coli incorporation, while extracellular Reactive Oxygen Species (ROS) production was determined by cytochrome c reduction assay, which was selected among the others due to its specificity for extracellular superoxide anion release. Phagocytic activity was significantly increased by EPA (P
- Subjects :
- Docosahexaenoic Acids
Neutrophils
Phagocytosis
Immunology
Biology
Pharmacology
chemistry.chemical_compound
Extracellular
Animals
chemistry.chemical_classification
Reactive oxygen species
General Veterinary
Superoxide
Goats
Cytochrome c
Eicosapentaenoic acid
Eicosapentaenoic Acid
chemistry
Biochemistry
Docosahexaenoic acid
biology.protein
Tetradecanoylphorbol Acetate
lipids (amino acids, peptides, and proteins)
Reactive Oxygen Species
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 131
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....38771f6faa2762f0b78937fd8fe5a5f2