Biosynthesis, molecular modeling and statistical optimization of xylanase from a mangrove associated actinobacterium Streptomyces variabilis (MAB3) using Box-Behnken design with its bioconversion efficacy

The present study was undertaken to evaluate the biosynthesis, molecular modeling and statistical optimization of xylanase production through Box-Behnken design by a mangrove associated actinobacterium Streptomyces variabilis (MAB3). Initially, the production of xylanase by the selected strain was carried through submerged fermentation using birchwood xylan as substrate. Further the xylanase production was statistically optimized through Box-Behnken design. It showed 5.30 fold increase of xylanase production by the isolate compared to ‘one factor at a time approach’ in the presence of the basal medium containing birchwood xylan (2.0% w/v) at pH 8.2, temperature 46.5 °C, inoculum size of 2% for 68 h. The analysis of variance (ANOVA) revealed high coefficient of determination (R2 = 0.9490) for the respective responses at significant level (P