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Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication
- Source :
- Cell Reports, Vol 25, Iss 2, Pp 464-477.e4 (2018), Cell reports
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- SUMMARY The urothelium is an epithelia barrier lined by a luminal layer of binucleated, octoploid, superficial cells. Superficial cells are critical for production and transport of uroplakins, a family of proteins that assemble into a waterproof crystalline plaque that helps protect against infection and toxic substances. Adult urothelium is nearly quiescent, but rapidly regenerates in response to injury. Yet the mechanism by which binucleated, polyploid, superficial cells are produced remains unclear. Here, we show that superficial cells are likely to be derived from a population of binucleated intermediate cells, which are produced from mononucleated intermediate cells via incomplete cytokinesis. We show that binucleated intermediate and superficial cells increase DNA content via endoreplication, passing through S phase without entering mitosis. The urothelium can be permanently damaged by repetitive or chronic injury or disease. Identification of the mechanism by which superficial cells are produced may be important for developing strategies for urothelial repair.<br />Graphical Abstract<br />In Brief Binucleated superficial cells are critical for urothelial barrier function. Wang et al. show that they derive from binucleated intermediate cells that form via incomplete cytokinesis. Both superficial and intermediate cells increase ploidy via endoreplication, a feature likely to be important for repair and response to environmental changes.
- Subjects :
- Male
0301 basic medicine
Population
Mitosis
Article
General Biochemistry, Genetics and Molecular Biology
Polyploidy
Mice
03 medical and health sciences
Polyploid
Uroplakins
Animals
Endoreduplication
Urothelium
education
lcsh:QH301-705.5
Cells, Cultured
Cell Proliferation
Cytokinesis
education.field_of_study
Chemistry
Regeneration (biology)
Cell Differentiation
3. Good health
Cell biology
030104 developmental biology
lcsh:Biology (General)
Female
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 25
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....383a430c82ad78dd7446b2e7f4668e2b