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Optimization of Cellulase and Xylanase Productions by Streptomyces thermocoprophilus Strain TC13W Using Oil Palm Empty Fruit Bunch and Tuna Condensate as Substrates
- Source :
- Applied Biochemistry and Biotechnology. 189:76-86
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The modified medium composed of the alkaline-pretreated oil palm empty fruit bunch (APEFB) and tuna condensate powder was used for cellulase and xylanase productions by Streptomyces thermocoprophilus strain TC13W. The APEFB contained 74.46% (w/w) cellulose, 15.72% (w/w) hemicellulose, and 6.40% (w/w) lignin. The tuna condensate powder contained 55.49% (w/w) protein and 11.05% (w/w) salt. In the modified medium with only 6.75 g/l tuna condensate powder, 10 g/l APEFB, and 0.5 g/l Tween 80, S. thermocoprophilus strain TC13W produced cellulase 4.9 U/ml and xylanase 9.0 U/ml. The enzyme productions in the modified medium were lower than cellulase (6.0 U/ml) and xylanase (12.0 U/ml) productions in the complex medium (CaCl2 0.1, MgSO4·7H2O 0.1, KH2PO4 0.5, K2HPO4 1.0, NaCl 0.2, yeast extract 5.0, NH4NO3 1.0, Tween 80 0.5). When tuna condensate powder in the modified medium was reduced to 5.0 g/l and Tween 80 was increased to 1.5 g/l, S. thermocoprophilus strain TC13W produced cellulase and xylanase activities of 9.1 and 12.1 U/ml, respectively. This study shows that the cost of enzyme production could be reduced by using pretreated EFB and tuna condensate as a carbon and a nitrogen source, respectively.
- Subjects :
- 0106 biological sciences
Bioengineering
Cellulase
Palm Oil
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
Substrate Specificity
chemistry.chemical_compound
010608 biotechnology
Streptomyces thermocoprophilus
Animals
Yeast extract
Lignin
Hemicellulose
Food science
Cellulose
Molecular Biology
biology
Tuna
010405 organic chemistry
General Medicine
Streptomyces
0104 chemical sciences
Xylosidases
chemistry
Xylanase
biology.protein
Biotechnology
Subjects
Details
- ISSN :
- 15590291 and 02732289
- Volume :
- 189
- Database :
- OpenAIRE
- Journal :
- Applied Biochemistry and Biotechnology
- Accession number :
- edsair.doi.dedup.....3831ccf15b61dcee0e2e30375e5ae88d