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Morphological differences between circulating tumor cells from prostate cancer patients and cultured prostate cancer cells
- Source :
- PLoS ONE, Vol 9, Iss 1, p e85264 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Circulating tumor cell (CTC) enumeration promises to be an important predictor of clinical outcome for a range of cancers. Established CTC enumeration methods primarily rely on affinity capture of cell surface antigens, and have been criticized for underestimation of CTC numbers due to antigenic bias. Emerging CTC capture strategies typically distinguish these cells based on their assumed biomechanical characteristics, which are often validated using cultured cancer cells. In this study, we developed a software tool to investigate the morphological properties of CTCs from patients with castrate resistant prostate cancer and cultured prostate cancer cells in order to establish whether the latter is an appropriate model for the former. We isolated both CTCs and cultured cancer cells from whole blood using the CellSearch® system and examined various cytomorphological characteristics. In contrast with cultured cancer cells, CTCs enriched by CellSearch® system were found to have significantly smaller size, larger nuclear-cytoplasmic ratio, and more elongated shape. These CTCs were also found to exhibit significantly more variability than cultured cancer cells in nuclear-cytoplasmic ratio and shape profile.
- Subjects :
- Male
Cytoplasm
Pathology
Anatomy and Physiology
Colorectal cancer
lcsh:Medicine
Cell Count
Cell Separation
Metastasis
Prostate cancer
Engineering
0302 clinical medicine
Circulating tumor cell
Molecular Cell Biology
Basic Cancer Research
Tumor Cells, Cultured
lcsh:Science
Aged, 80 and over
0303 health sciences
Multidisciplinary
Prostate Cancer
Prostate
Prostate Diseases
Middle Aged
Neoplastic Cells, Circulating
Prognosis
Cellular Structures
Prostatic Neoplasms, Castration-Resistant
Oncology
Organ Specificity
030220 oncology & carcinogenesis
Medicine
Research Article
Cell Physiology
medicine.medical_specialty
Urology
Biomedical Engineering
Bioengineering
Biology
03 medical and health sciences
Breast cancer
Antigen
Biomarkers, Tumor
medicine
Humans
Aged
030304 developmental biology
Cell Nucleus
lcsh:R
medicine.disease
Cell culture
Cancer cell
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3829a40b3a1c46984fd5bc1de171f83e