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Molecular cloning and transcriptional regulation of two γ-carbonic anhydrase genes in the green macroalga Ulva prolifera
- Source :
- Genetica. 149:63-72
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Ulva prolifera O.F. Müller (Ulvophyceae, Chlorophyta) is well known as a typical green-tide forming macroalga which has caused the world's largest macroalgal blooms in the Yellow Sea of China. In this study, two full-length γ-carbonic anhydrase (γ-CA) genes (UpγCA1 and UpγCA2) were cloned from U. prolifera. UpγCA1 has three conserved histidine residues, which act as an active site for binding a zinc metal ion. In UpγCA2, two of the three histidine residues were replaced by serine and arginine, respectively. The two γ-CA genes are clustered together with other γ-CAs in Chlorophyta with strong support value (100% bootstrap) in maximum likelihood (ML) phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analysis showed that stressful environmental conditions markedly inhibited transcription levels of these two γ-CA genes. Low pH value (pH 7.5) significantly increased transcription level of UpγCA2 not UpγCA1 at 12 h, whereas high pH value (pH 8.5) significantly inhibited the transcription of these two γ-CA genes at 6 h. These findings enhanced our understanding on transcriptional regulation of γ-CA genes in response to environmental factors in U. prolifera.
- Subjects :
- 0106 biological sciences
0301 basic medicine
China
Carbonic Anhydrase I
Transcription, Genetic
Plant Science
Chlorophyta
Carbonic Anhydrase II
010603 evolutionary biology
01 natural sciences
Ulva
03 medical and health sciences
Transcription (biology)
Carbonic anhydrase
Genetics
Transcriptional regulation
Cloning, Molecular
Gene
Phylogeny
Histidine
biology
Ulvophyceae
Ulva prolifera
General Medicine
biology.organism_classification
030104 developmental biology
Gene Expression Regulation
Biochemistry
Insect Science
biology.protein
Animal Science and Zoology
Subjects
Details
- ISSN :
- 15736857 and 00166707
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- Genetica
- Accession number :
- edsair.doi.dedup.....381f0bdfa684070d21a173e7ce2f5aca