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Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns
- Source :
- Comparative Biochemistry and Physiology-Part D: Genomics and Proteomics, 25(March), 26-33. Elsevier B.V.
- Publication Year :
- 2018
-
Abstract
- While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P < 0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.
- Subjects :
- 0106 biological sciences
Physiology
Rotifera
Gene Expression
Fresh Water
010501 environmental sciences
urologic and male genital diseases
01 natural sciences
Biochemistry
Genome
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Databases, Genetic
Gene expression
Brachionus calyciflorus
Benzo(a)pyrene
Genetics
Animals
heterocyclic compounds
Amino Acid Sequence
Molecular Biology
Gene
Phylogeny
0105 earth and related environmental sciences
Sequence Homology, Amino Acid
biology
Sequence Analysis, RNA
Ecology
010604 marine biology & hydrobiology
organic chemicals
Cytochrome P450
Brachionus
respiratory system
biology.organism_classification
enzymes and coenzymes (carbohydrates)
chemistry
international
biology.protein
Xenobiotic
Water Pollutants, Chemical
Subjects
Details
- Language :
- English
- ISSN :
- 1744117X
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology-Part D: Genomics and Proteomics, 25(March), 26-33. Elsevier B.V.
- Accession number :
- edsair.doi.dedup.....3812b110f18eabc39454c608caa7c0ed