Back to Search
Start Over
Mixture Effects of Diesel Exhaust and Metal Oxide Nanoparticles in Human Lung A549 Cells
- Source :
- Nanomaterials, Nanomaterials, Vol 9, Iss 9, p 1302 (2019), Volume 9, Issue 9
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Airborne ultrafine particles (UFP) mainly derive from combustion sources (e.g., diesel exhaust particles&mdash<br />DEP), abrasion sources (non-exhaust particles) or from the unintentional release of engineered nanoparticles (e.g., metal oxide nanoparticles&mdash<br />NPs), determining human exposure to UFP mixtures. The aim of the present study was to analyse the combined in vitro effects of DEP and metal oxide NPs (ZnO, CuO) on human lung A549 cells. The mixtures and the relative single NPs (DEP, ZnO, CuO) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and inductively coupled plasma-optic emission spectroscopy (ICP-OES). Cells were exposed for different times (3&ndash<br />72 h) to mixtures of standard DEP at a subcytotoxic concentration and ZnO and CuO at increasing concentrations. At the end of the exposure, the cytotoxicity was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and clonogenic tests, the pro-inflammatory potential was evaluated by interleukin-8 (IL-8) release and the cell morphology was investigated by fluorescence and transmission electron microscopy. The obtained results suggest that the presence of DEP may introduce new physico-chemical interactions able to increase the cytotoxicity of ZnO and to reduce that of CuO NPs.
- Subjects :
- Materials science
Diesel exhaust
General Chemical Engineering
Oxide
010501 environmental sciences
Cell morphology
01 natural sciences
complex mixtures
metal oxide nanoparticles
Article
Metal
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
Dynamic light scattering
Bromide
Ultrafine particle
General Materials Science
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
metal oxide nanoparticle
diesel exhaust
respiratory system
3. Good health
ultrafine particles
mixture
mixtures
chemistry
lcsh:QD1-999
13. Climate action
Transmission electron microscopy
visual_art
visual_art.visual_art_medium
cytotoxicity
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 20794991
- Volume :
- 9
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Nanomaterials
- Accession number :
- edsair.doi.dedup.....380e857bcebcb8fb1c6f4246f79bf8bd