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Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of glycyrrhizic acid
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Glycyrrhizic acid (GL) is a major active compound of licorice. The specific monoclonal antibody (MAb) (designated as 8F8A8H42H7) against GL was produced with the immunogen GL–BSA conjugate. The dissociation constant (K d) value of the MAb was approximately 9.96×10−10 M. The cross reactivity of the MAb with glycyrrhetic acid was approximately 2.6%. The conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA adapted with a modified procedure were established using the MAb. The IC50 value and the detect range by the conventional icELISA were 1.1 ng mL−1 and 0.2–5.1 ng mL−1, respectively. The IC50 value and the detect range by the simplified icELISA were 5.3 ng mL−1 and 1.2–23.8 ng mL−1, respectively. The two icELISA formats were used to analyze GL contents in the roots of wild licorice and different parts of cultivated licorice (Glycyrrhiza uralensis Fisch). The results obtained with the two icELISAs agreed well with those of the HPLC analysis. The correlation coefficient was more than 0.98 between HPLC and the two icELISAs. The two icELISAs were shown to be appropriate, simple, and effective for the quality control of raw licorice root materials.
- Subjects :
- Monoclonal antibody
Immunogen
medicine.drug_class
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Biochemistry
Cross-reactivity
High-performance liquid chromatography
Analytical Chemistry
Licorice
Mice
medicine
Animals
IC50
Chromatography, High Pressure Liquid
Original Paper
Mice, Inbred BALB C
Chromatography
Molecular Structure
biology
Chemistry
Glycyrrhiza uralensis
Antibodies, Monoclonal
Glycyrrhizic Acid
biology.organism_classification
Dissociation constant
ELISA
Female
Conjugate
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 386
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....37d9c2b83186af61aeba83a2546816ba