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Knockout of Murine Mamld1 Impairs Testicular Growth and Daily Sperm Production but Permits Normal Postnatal Androgen Production and Fertility
- Source :
- International Journal of Molecular Sciences, Vol 18, Iss 6, p 1300 (2017), International Journal of Molecular Sciences; Volume 18; Issue 6; Pages: 1300, International Journal of Molecular Sciences
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- MAMLD1 has been implicated in testicular function in both human and mouse fetuses. Although three patients with MAMLD1 mutations were reported to have hypergonadotropic hypogonadism in their teens, the functional significance of MAMLD1 in the postnatal testis remains unclear. Here, we analyzed the phenotype of Mamld1 knockout (KO) male mice at reproductive ages. The reproductive organs of KO male mice were morphologically unremarkable, except for relatively small testes. Seminiferous tubule size and number of proliferating spermatogonia/spermatocytes were reduced in the KO testis. Daily sperm production of KO mice was mildly attenuated, whereas total sperm counts in epididymal semen remained normal. Sperm motility and morphology, as well as androgen levels in serum and testicular tissues and the number of pups born from cross-mated wildtype (WT) female mice, were comparable between WT and KO male mice. These results indicate that MAMLD1 contributes to the maintenance of postnatal testicular growth and daily sperm production but is dispensable for androgen biosynthesis and fertility. MAMLD1 likely plays supporting roles in multiple and continuous steps of male reproduction.
- Subjects :
- 0301 basic medicine
Male
Serum
Time Factors
lcsh:Chemistry
Mice
Spermatocytes
Testosterone
lcsh:QH301-705.5
Spectroscopy
Sperm motility
Mice, Knockout
Sperm Count
Gene Expression Regulation, Developmental
Dihydrotestosterone
General Medicine
Seminiferous Tubules
Spermatozoa
Computer Science Applications
Seminiferous tubule
medicine.anatomical_structure
Phenotype
Knockout mouse
Androgens
Sperm Motility
Female
medicine.drug
medicine.medical_specialty
endocrine system
medicine.drug_class
androgen
Biology
testis
Catalysis
Article
knockout mouse
mutation
reproduction
Inorganic Chemistry
Andrology
03 medical and health sciences
Hypergonadotropic hypogonadism
Internal medicine
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Models, Genetic
urogenital system
Organic Chemistry
Androstenedione
Androgen
medicine.disease
Spermatogonia
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
Fertility
lcsh:Biology (General)
lcsh:QD1-999
Microscopy, Electron, Scanning
Spermatogenesis
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....37d37c518bab0b4db706101e092f6e8b